The original CyberKnife was developed at Stanford, where the first patient was treated in 1994.

The prototype unit was used between 1994 and 2001. The CyberKnife was approved by the FDA (U.S. Food and Drug Administration) in 2001, and the first FDA approved CyberKnife was installed at Stanford in October, 2001.

• The 1,000th CyberKnife patient at Stanford was treated in October 2003. 
  
  
• The 2,000th CyberKnife patient was treated in November 2005.
  
  
• The 3,500th CyberKnife patient has undergone CyberKnife treatment in 2007.
  
  
• Stanford is widely regarded as the most experienced CyberKnife treatment center in the world.
• Stanford opened its second CyberKnife in October of 2006 (the first institution in the world to have two CyberKnife units in operation).